We are developing and improving the structural biology research environment
using the high-brilliance synchrotron radiation at SPring-8.

For those who have an accepted proposal, Beamtime survey for 2024A (Non-proprietary research proposal)

From 2021B, Cryo-TEM and Bio-SAXS have become available. Please apply by following the steps below.
This guidance is for “Non-proprietary research proposal”. If you have “Proprietary research proposal”, please click here.

Common subject matter

  1. Shifts will be decided according to the rating of your proposal when it was reviewed.
  2. If there is unallocated beamtime(machine time) after the allocation of each period, there will be a call for additional beamtime(machine time). Please refer to the announcement.
  3. Please note that the proposal numbers of the proposals that were accepted in 2023B have been changed. The number can be checked at the UI siteUI site.


PX-Beamline (BL41XU, BL45XU, BL32XU, BL26B1)

The deadlines for the two periods of 2024A are as follows.
 1st period (10am April 8th to 10am June 9th): Finished
 2nd period (10am June 9th to 10am July 31st): The deadline is May 8, 2024 at 10:00 (JST)
Please refer to the following for a detailed beamtime Schedule

If you wish to use a beamline during the two periods, please fill “Survey_Sheet” and “Spec_Sheet” and upload them from the application page. If you have a photo of the crystal, please upload it. We will inform the principal investigator by email the username and password of the application page. If there are multiple assignments, please upload each.

PX-BLs Specifications
Beamtime Survey Sheet
Crystal Spec Sheet
Beamtime Schedule


  1. Automatic data collection (with or without a visit) can be used at BL45XU and BL32XU. Since international travel is currently very difficult, please consider using automatic data collection without a visit. Please note that from October 2020, the operation of BL45XU and BL32XU will be changed to be ready for the operation of the Uni-puck automatic exchange system.
  2. Requests for non-proprietary beamtime that cannot be allocated to BL45XU, may be allocated to BL41XU, taking into account the content of the experiment and its grade.
  3. Remote access can be used at BL41XU. Please contact the beamline staff for details » stbio (followed by @spring8.or.jp).
  4. You can send three requests with priorities. In each request, only one beamline can be selected from BL41XU, BL45XU, BL32XU, BL26B1.
  5. The shortest unit of beamtime is 0.25 shifts [2 hours] for BL32XU (Only measurement without visiting) and BL41XU and BL45XU, 1.5 shifts [12 hours] for BL32XU(Visiting measurement), and 1 shift [8 hours] for BL26B1.
  6. Date of measurement will be decided after arrangement.


CryoTEM

Please check here for the public use of CryoTEM.

The deadlines for the two periods of 2024A are as follows.
 1st period (10am April 1st to 10am June 9th): Finished
 2nd period (10am June 9th to 10am August 1st): The deadline is May 8, 2024 at 10:00 (JST)
Please refer to the following for a detailed beamtime Schedule

If you wish to use a beamline during the two periods, please fill “Survey_Sheet” and “Spec_Sheet” and upload them from the application page. If you have a photo of the sample with an electron microscope, please upload it. We will inform the principal investigator by email the username and password of the application page. If there are multiple assignments, please upload each.
The measurement date will be adjusted and notified after the number of shifts is determined.

The pre-use training course is required to use the CryoTEM either through the regular workshop or your own machine time. If you wish to have machine time before taking the course, please indicate so in the special notes.

Information about CryoTEM
Machinetime Survey Sheet
Sample Spec Sheet
Machinetime schedule

Bio-SAXS

SEC-SAXS or Static measurements of protein solution samples is available.

The deadlines for the two periods of 2024A are as follows.
 1st period (10am April 8th to 10am June 9th): Finished
 2nd period (10am June 9th to 10am July 31st): The deadline is May 8, 2024 at 10:00 (JST)

If you wish to use a beamline during the two periods, please fill “Survey_Sheet” and “Spec_Sheet” and upload them from the application page. If you have gel filtration profile data of the sample, please upload it. We will inform the principal investigator by email the username and password of the application page. If there are multiple assignments, please upload each.

The measurement date will be adjusted and notified after the number of shifts is determined.
Information about Bio-SAXS
Machinetime Survey Sheet
Sample Spec Sheet

Contact

e-mail (followed by @spring8.or.jp)
Contactstbio

*The contact email address has changed since June 2022.

SPring-8 Program Review Committee, SB Subcommittee